Hotspot

ndhI-ndhG

SSC · 125771-126141 · 371 bp

Back to Nekemias

Pi 0.00854
MarkerSeek score 59.80
Primer available yes
Label ndhI-ndhG

Feature Metadata

label_name
ndhI-ndhG
feature_type
igs
region
SSC
length_bp
371
variable_sites
6
pi
0.00854
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhG_p1 TCAAATTGCTTTGGGTCGTT CCACTCCTAGACCTCCCAAT 40.0 55.0 56.4 57.8 532-533 bp 1.0 67.0
ndhI-ndhG_p2 TCAAATTGCTTTGGGTCGTT TACCACTCCTAGACCTCCCA 40.0 55.0 56.4 58.3 534-535 bp 1.0 66.0
ndhI-ndhG_p3 ATCAAATTGCTTTGGGTCGT CCACTCCTAGACCTCCCAAT 40.0 55.0 56.2 57.8 533-534 bp 1.0 65.1
ndhI-ndhG_p4 TCAAATTGCTTTGGGTCGTTT CCACTCCTAGACCTCCCAAT 38.1 55.0 57.2 57.8 532-533 bp 1.0 65.0
ndhI-ndhG_p5 TCCACTTATGATCGTCACGA CCACTCCTAGACCTCCCAAT 45.0 55.0 56.1 57.8 564-565 bp 1.0 64.2