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psbT-psbN

LSC · 79734-79816 · 83 bp

Back to Nekemias

Pi 0.0382

MarkerSeek score 70.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 83
variable_sites: 6
pi: 0.0382
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	ATCCATTTGAGGAACACGGG	50.0	50.0	57.6	57.9	142-143 bp	1.0	80.0
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	ATCCATTTGAGGAACACGGG	50.0	50.0	57.5	57.9	140-141 bp	1.0	79.2
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	GCAGCCCTCTCAACAACTAA	50.0	50.0	57.6	57.8	165-166 bp	1.0	79.0
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	TTTGAGGAACACGGGGACTA	50.0	50.0	57.6	58.3	137-138 bp	1.0	79.0
psbT-psbN_p5	TCGAGAACCGCCTAAAGTTC	GGTACGCCTTATATACCGCC	50.0	55.0	57.6	57.7	190-191 bp	1.0	77.8