Hotspot

accD-psaI

LSC · 63381-64135 · 755 bp

Back to Nekemias

Pi 0.00332

MarkerSeek score 61.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 5
pi: 0.00332
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTTCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.9	58.0	866-868 bp	1.0	71.9
accD-psaI_p2	GCTTCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.9	57.9	872-874 bp	1.0	71.3
accD-psaI_p3	GCTTCACGCTTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.6	58.0	866-868 bp	1.0	69.6
accD-psaI_p4	GCTTCACGCTTTCTTTCCTTT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.6	57.9	872-874 bp	1.0	69.0
accD-psaI_p5	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	892-894 bp	1.0	66.8