Hotspot

psbZ-trnG-GCC

LSC · 39546-40230 · 685 bp

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Pi 0.00706
MarkerSeek score 50.30
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
685
variable_sites
8
pi
0.00706
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 853-899 bp 1.0 78.7
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 854-900 bp 1.0 78.7
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 851-897 bp 1.0 78.3
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GAATCGAACCCGCATCTTCT 55.0 50.0 58.3 58.1 852-898 bp 1.0 78.3
psbZ-trnG-GCC_p5 TTGCTTCTCCTGATGGTTGG GCATTTCTTTACCTTGGGCG 50.0 50.0 57.8 57.7 882-928 bp 1.0 76.9