Hotspot

rps19

IRa · 159077-159355 · 279 bp

Back to Neillia

Pi 0.0155

MarkerSeek score 39.90

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRa
length_bp: 279
variable_sites: 6
pi: 0.0155
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.39
markerseek_score: 39.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTGATTCTTCGTCGCCGTAG	AGGCAAATGCTCCTTTCCAT	50.0	45.0	58.1	57.8	274 bp	0.4	53.3
rps19_p2	ATTTGATTCTTCGTCGCCGT	AGGCAAATGCTCCTTTCCAT	45.0	45.0	58.3	57.8	276 bp	0.4	51.8
rps19_p3	TTTGATTCTTCGTCGCCGTA	AGGCAAATGCTCCTTTCCAT	45.0	45.0	57.6	57.8	275 bp	0.4	51.2
rps19_p4	CGTCGCCGTAGTAAATAGGC	AGGCAAATGCTCCTTTCCAT	55.0	45.0	58.6	57.8	265 bp	0.4	49.3
rps19_p5	GATTCTTCGTCGCCGTAGTA	AGGCAAATGCTCCTTTCCAT	50.0	45.0	57.3	57.8	272 bp	0.4	48.1