Hotspot

ndhE-ndhG

SSC · 122240-122476 · 237 bp

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Pi 0.0165

MarkerSeek score 62.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 237
variable_sites: 9
pi: 0.0165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTTCGGCTCGTGATCAATCC	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.3	58.2	344-351 bp	1.0	82.0
ndhE-ndhG_p2	CGGCTCGTGATCAATCCATA	TTGATAGGTGCAATTGCCGT	50.0	45.0	57.6	58.2	341-348 bp	1.0	81.3
ndhE-ndhG_p3	TTTCGGCTCGTGATCAATCC	TTAGCCGCTTTGATAGGTGC	50.0	50.0	58.3	58.3	353-360 bp	1.0	80.6
ndhE-ndhG_p4	CGGCTCGTGATCAATCCATA	TTAGCCGCTTTGATAGGTGC	50.0	50.0	57.6	58.3	350-357 bp	1.0	79.8
ndhE-ndhG_p5	AACCATATTTCGGCTCGTGA	TTGATAGGTGCAATTGCCGT	45.0	45.0	57.3	58.2	351-358 bp	1.0	79.2