Hotspot

ccsA-ndhD

SSC · 119536-119817 · 282 bp

Back to Neillia

Pi 0.022

MarkerSeek score 61.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 282
variable_sites: 14
pi: 0.022
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTGCAATTGTGGCCTCAATG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	442 bp	1.0	81.5
ccsA-ndhD_p2	CTGCAATTGTGGCCTCAATG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	58.0	58.1	443 bp	1.0	81.4
ccsA-ndhD_p3	ATTCTGCAATTGTGGCCTCA	CAGTTGACAAGGTCGAAGCT	45.0	50.0	58.1	58.1	445 bp	1.0	80.9
ccsA-ndhD_p4	TCTGCAATTGTGGCCTCAAT	CAGTTGACAAGGTCGAAGCT	45.0	50.0	58.1	58.1	443 bp	1.0	80.9
ccsA-ndhD_p5	TCTGCAATTGTGGCCTCAAT	TCAGTTGACAAGGTCGAAGC	45.0	50.0	58.1	58.1	444 bp	1.0	80.9