Hotspot

rpl32-trnL-UAG

SSC · 117465-118395 · 931 bp

Back to Neillia

Pi 0.00892

MarkerSeek score 55.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 931
variable_sites: 17
pi: 0.00892
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGATATCGGGCAGCATTGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1033-1050 bp	1.0	82.5
rpl32-trnL-UAG_p2	GGATATCGGGCAGCATTGAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1034-1051 bp	1.0	82.5
rpl32-trnL-UAG_p3	TTAAGGGAGAGAGGGGTTGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.0	58.0	1423-1440 bp	1.0	82.2
rpl32-trnL-UAG_p4	TTAAGGGAGAGAGGGGTTGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.0	58.0	1424-1441 bp	1.0	82.2
rpl32-trnL-UAG_p5	GGAGAGAGGGGTTGGGTTAT	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	1418-1435 bp	1.0	81.6