Hotspot

petA-psbJ

LSC · 65968-66917 · 950 bp

Back to Neillia

Pi 0.00611

MarkerSeek score 51.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 12
pi: 0.00611
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1069-1097 bp	1.0	72.5
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1070-1098 bp	1.0	72.5
petA-psbJ_p3	TGCTCTTCTTGGCATCTGTT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.4	57.4	1068-1096 bp	1.0	72.0
petA-psbJ_p4	TTGCTCTTCTTGGCATCTGT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.4	57.4	1069-1097 bp	1.0	72.0
petA-psbJ_p5	CGTGTCCAAGGTCTTTTGCT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.7	57.5	1085-1113 bp	1.0	71.6