Hotspot

trnM-CAU-atpE

LSC · 55415-55609 · 195 bp

Back to Neillia

Pi 0.0174

MarkerSeek score 65.50

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 195
variable_sites: 8
pi: 0.0174
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	294 bp	1.0	78.6
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.4	58.1	286 bp	1.0	78.4
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	ACTTGAGGAAAGCTGAAGGC	50.0	50.0	58.4	58.1	342 bp	1.0	77.8
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	CTTGAGGAAAGCTGAAGGCA	50.0	50.0	58.4	58.1	341 bp	1.0	77.8
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	TGAGGAAAGCTGAAGGCAAG	50.0	50.0	58.4	58.1	339 bp	1.0	77.8