Hotspot

psbZ-trnG-GCC

LSC · 38204-38630 · 427 bp

Back to Neillia

Pi 0.0262
MarkerSeek score 69.40
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
427
variable_sites
22
pi
0.0262
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGTCTTTCTGGTGGGTATCCT AATCGAACCCGCATCTTCTC 47.6 50.0 57.8 58.1 525-665 bp 1.0 76.8
psbZ-trnG-GCC_p2 AGTCTTTCTGGTGGGTATCCT GAATCGAACCCGCATCTTCT 47.6 50.0 57.8 58.1 526-666 bp 1.0 76.8
psbZ-trnG-GCC_p3 AGTCTTTCTGGTGGGTATCC AATCGAACCCGCATCTTCTC 50.0 50.0 56.2 58.1 525-665 bp 1.0 72.3
psbZ-trnG-GCC_p4 GTCTTTCTGGTGGGTATCCT AATCGAACCCGCATCTTCTC 50.0 50.0 56.2 58.1 524-664 bp 1.0 72.3
psbZ-trnG-GCC_p5 GTCTTTCTGGTGGGTATCCT GAATCGAACCCGCATCTTCT 50.0 50.0 56.2 58.1 525-665 bp 1.0 72.3