Hotspot

trnK-UUU-rps16

LSC · 4253-5170 · 918 bp

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Pi 0.00589
MarkerSeek score 63.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
918
variable_sites
12
pi
0.00589
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1049-1056 bp 1.0 79.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1048-1055 bp 1.0 79.4
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 1058-1065 bp 1.0 78.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 1053-1060 bp 1.0 77.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 1052-1059 bp 1.0 77.8