Hotspot

ndhD

SSC · 108704-110206 · 1503 bp

Back to Nassella

Pi 0.000887

MarkerSeek score 56.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 2
pi: 0.000887
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGGTTCTCAAAAATTCGAGA	CGGGCTTTTCTGGTCAAAAC	38.1	50.0	55.1	57.9	1652 bp	1.0	48.2
ndhD_p2	AGGGTTCTCAAAAATTCGAGA	GGGCTTTTCTGGTCAAAACG	38.1	50.0	55.1	57.9	1651 bp	1.0	48.2
ndhD_p3	AGGGTTCTCAAAAATTCGAGA	ACGGGCTTTTCTGGTCAAAA	38.1	45.0	55.1	58.2	1653 bp	1.0	47.4
ndhD_p4	CAAAGGGTTCTCAAAAATTCGAGA	CGGGCTTTTCTGGTCAAAAC	37.5	50.0	57.9	57.9	1655 bp	1.0	46.4
ndhD_p5	CAAAGGGTTCTCAAAAATTCGAGA	GGGCTTTTCTGGTCAAAACG	37.5	50.0	57.9	57.9	1654 bp	1.0	46.4