Hotspot

atpI

LSC · 31520-32263 · 744 bp

Back to Nassella

Pi 0.000896

MarkerSeek score 32.40

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 1
pi: 0.000896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GAGGGCCGTTCTCTCTCTAT	TTCTGGCTGTCTCGCAATAC	55.0	50.0	58.0	58.0	1688-1694 bp	1.0	80.2
atpI_p2	GAGGGCCGTTCTCTCTCTAT	AACCCGCACAAAAGATGACT	55.0	45.0	58.0	58.0	1275-1281 bp	1.0	80.2
atpI_p3	ATCAGCTGCAATAAGGGCTC	TTCTGGCTGTCTCGCAATAC	50.0	50.0	58.0	58.0	2112-2118 bp	1.0	80.0
atpI_p4	AATTGTGACCCAGATCTCGC	TTCTGGCTGTCTCGCAATAC	50.0	50.0	58.0	58.0	1787-1793 bp	1.0	80.0
atpI_p5	ATCAGCTGCAATAAGGGCTC	AACCCGCACAAAAGATGACT	50.0	45.0	58.0	58.0	1699-1705 bp	1.0	80.0