Hotspot

ndhF-rpl32

SSC · 105588-106490 · 903 bp

Back to Nassella

Pi 0.00148
MarkerSeek score 59.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
903
variable_sites
2
pi
0.00148
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TAAGGCCAAACCCCATTGAC TCCAAAGATTTTTACGAATACGCT 50.0 33.3 58.1 57.3 1043 bp 1.0 41.8
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 1014 bp 1.0 41.4
ndhF-rpl32_p3 GGCCAAACCCCATTGACATA TCCAAAGATTTTTACGAATACGCT 50.0 33.3 58.1 57.3 1040 bp 1.0 41.2
ndhF-rpl32_p4 TAAGGCCAAACCCCATTGAC TCCAAAGATTTTTACGAATACGC 50.0 34.8 58.1 55.9 1043 bp 1.0 40.2
ndhF-rpl32_p5 TAAGGCCAAACCCCATTGAC CCAAAGATTTTTACGAATACGCT 50.0 34.8 58.1 55.9 1042 bp 1.0 40.2