Hotspot

petA-psbJ

LSC · 61579-62418 · 840 bp

Back to Nassella

Pi 0.00159

MarkerSeek score 59.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 2
pi: 0.00159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTCCTGTGATTGGT	50.0	47.6	57.9	58.0	1017 bp	1.0	71.7
petA-psbJ_p2	TGTTCTTCTTCGCATCCGTT	GCTGGTATTCCTGTGATTGGT	45.0	47.6	57.8	58.0	1010 bp	1.0	70.8
petA-psbJ_p3	TTGTTCTTCTTCGCATCCGT	GCTGGTATTCCTGTGATTGGT	45.0	47.6	57.8	58.0	1011 bp	1.0	70.8
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	AGCTGGTATTCCTGTGATTGG	50.0	47.6	57.9	57.7	1018 bp	1.0	69.4
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGGCTGATAGGTACTGTAGCT	50.0	47.6	57.9	57.7	1035 bp	1.0	69.2