Hotspot

rbcL-psaI

LSC · 56697-58288 · 1592 bp

Back to Nassella

Pi 0.00126

MarkerSeek score 59.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1592
variable_sites: 3
pi: 0.00126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TTCGAGCCGGTAGATACTGT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1689-1690 bp	1.0	79.2
rbcL-psaI_p2	TTCGAGCCGGTAGATACTGT	TCATTGCAATTGCCGGAAAC	50.0	45.0	58.0	57.9	1688-1689 bp	1.0	79.2
rbcL-psaI_p3	TGCCAAATGGAGTCCTGAAC	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.1	57.9	1750-1751 bp	1.0	78.8
rbcL-psaI_p4	TGCCAAATGGAGTCCTGAAC	TCATTGCAATTGCCGGAAAC	50.0	45.0	58.1	57.9	1749-1750 bp	1.0	78.8
rbcL-psaI_p5	TTCGAGCCGGTAGATACTGT	ATTGCAATTGCCGGAAACAC	50.0	45.0	58.0	58.2	1686-1687 bp	1.0	78.2