Hotspot

ndhC-trnV-UAC

LSC · 50655-51530 · 876 bp

Back to Nassella

Pi 0
MarkerSeek score 59.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
876
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 995-1002 bp 1.0 79.7
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 996-1003 bp 1.0 77.8
ndhC-trnV-UAC_p3 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 994-1001 bp 1.0 77.2
ndhC-trnV-UAC_p4 CACTAACCGGGGCTAAAAGT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 997-1004 bp 1.0 76.1
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT CGGTTCGAATCCGTATAGCC 50.0 55.0 58.3 58.3 995-1002 bp 1.0 75.4