Hotspot

trnF-GAA-ndhJ

LSC · 48383-48977 · 595 bp

Back to Nassella

Pi 0.00112

MarkerSeek score 59.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 1
pi: 0.00112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	704-706 bp	1.0	78.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	694-696 bp	1.0	78.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TCCACGCCTTAAACGTATCC	50.0	50.0	58.1	57.7	731-733 bp	1.0	77.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCACGCCTTAAACGTATCCT	50.0	50.0	58.1	57.7	730-732 bp	1.0	77.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	735-737 bp	1.0	76.6