Hotspot

trnD-GUC-psbM

LSC · 16364-17135 · 772 bp

Back to Nassella

Pi 0.000864

MarkerSeek score 62.40

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 1
pi: 0.000864
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	50.0	45.0	58.5	55.8	863-883 bp	1.0	60.4
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	AGAATGAACAGTGCAGTAGC	55.0	45.0	58.7	55.8	895-915 bp	1.0	58.9
trnD-GUC-psbM_p3	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.0	55.8	864-884 bp	1.0	56.2
trnD-GUC-psbM_p4	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.9	55.8	877-897 bp	1.0	55.0
trnD-GUC-psbM_p5	TCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.8	55.8	862-882 bp	1.0	54.3