Hotspot

trnG-UCC-trnT-GGU

LSC · 13595-15123 · 1529 bp

Back to Nassella

Pi 0.000872
MarkerSeek score 46.40
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1529
variable_sites
2
pi
0.000872
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGACTTACGTCTTACCACGC 38.1 50.0 55.5 57.7 1679 bp 1.0 49.1
trnG-UCC-trnT-GGU_p2 AGAACGAATCACACTTTTACCAC TGACTTACGTCTTACCACGC 39.1 50.0 57.3 57.7 1682 bp 1.0 48.1
trnG-UCC-trnT-GGU_p3 GAACGAATCACACTTTTACCACT TGACTTACGTCTTACCACGC 39.1 50.0 57.3 57.7 1681 bp 1.0 48.1
trnG-UCC-trnT-GGU_p4 AACGAATCACACTTTTACCACT TGACTTACGTCTTACCACGC 36.4 50.0 56.2 57.7 1680 bp 1.0 47.2
trnG-UCC-trnT-GGU_p5 ACGAATCACACTTTTACCACT GACTTACGTCTTACCACGCT 38.1 50.0 55.5 57.4 1678 bp 1.0 46.8