Hotspot

ycf1

SSC · 126886-132600 · 5715 bp

Pi 0.0546

MarkerSeek score 73.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5715
variable_sites: 298
pi: 0.0546
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACGAATCTGATGCTCCACC	TTGCGAGAGAACCAAGATCG	50.0	50.0	58.0	58.0	258-264 bp	1.0	89.6
ycf1_p2	TCCCACGCTTCTGCTATTTC	TTGCGAGAGAACCAAGATCG	50.0	50.0	58.0	58.0	703-718 bp	1.0	89.3
ycf1_p3	CCCACGCTTCTGCTATTTCT	TTGCGAGAGAACCAAGATCG	50.0	50.0	58.0	58.0	702-717 bp	1.0	89.3
ycf1_p4	GTATCAACGCCACTTCGTCT	TTGCGAGAGAACCAAGATCG	50.0	50.0	58.0	58.0	372 bp	0.5	59.9
ycf1_p5	CACCGTGACGTCTTCTTGAT	TTGCGAGAGAACCAAGATCG	50.0	50.0	57.9	58.0	248 bp	0.5	59.4