Hotspot

rpl32-trnL-UAG

SSC · 116425-117223 · 799 bp

Back to Nasa

Pi 0.0748
MarkerSeek score 77.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
799
variable_sites
59
pi
0.0748
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTCCTTGAGCTTGCCCAATT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1442-1734 bp 1.0 85.8
rpl32-trnL-UAG_p2 TTCCTTGAGCTTGCCCAATT GGAGAGCGGGGACTAATAGT 45.0 55.0 58.0 58.0 1985 bp 0.5 59.9
rpl32-trnL-UAG_p3 TTTCCTTGAGCTTGCCCAAT GGAGAGCGGGGACTAATAGT 45.0 55.0 58.0 58.0 1986 bp 0.5 59.9
rpl32-trnL-UAG_p4 TTCCTTGAGCTTGCCCAATT TGGAGAGCGGGGACTAATAG 45.0 55.0 58.0 58.0 1986 bp 0.5 59.9
rpl32-trnL-UAG_p5 TTTCCTTGAGCTTGCCCAAT TGGAGAGCGGGGACTAATAG 45.0 55.0 58.0 58.0 1987 bp 0.5 59.9