Hotspot

ndhF-rpl32

SSC · 115224-116262 · 1039 bp

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Pi 0.0784
MarkerSeek score 78.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1039
variable_sites
80
pi
0.0784
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCGGAAAAAGAAGAAGCCCC CAAGTGGGTTGATGGGGAAA 50.0 50.0 58.1 58.0 1619-1818 bp 1.0 85.1
ndhF-rpl32_p2 ACGAAAGGTATGATCCACGC CAAGTGGGTTGATGGGGAAA 50.0 50.0 58.1 58.0 1569 bp 0.5 59.4
ndhF-rpl32_p3 CGAAAGGTATGATCCACGCA CAAGTGGGTTGATGGGGAAA 50.0 50.0 58.1 58.0 1568 bp 0.5 59.4
ndhF-rpl32_p4 ACGAAAGGTATGATCCACGC TGTGACAAGTGGGTTGATGG 50.0 50.0 58.1 58.0 1574 bp 0.5 59.3
ndhF-rpl32_p5 CGAAAGGTATGATCCACGCA TGTGACAAGTGGGTTGATGG 50.0 50.0 58.1 58.0 1573 bp 0.5 59.3