Hotspot

trnW-CCA-trnP-UGG

LSC · 69041-69204 · 164 bp

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Pi 0.0513
MarkerSeek score 78.20
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
164
variable_sites
8
pi
0.0513
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT ATTGAAGGGATGTAGCGCAG 50.0 50.0 58.0 58.0 282-284 bp 1.0 83.9
trnW-CCA-trnP-UGG_p2 GAAGAACGGAATCACGCTCT ATTGAAGGGATGTAGCGCAG 50.0 50.0 58.0 58.0 327-329 bp 1.0 83.6
trnW-CCA-trnP-UGG_p3 AGAAGAACGGAATCACGCTC GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 288-290 bp 1.0 83.6
trnW-CCA-trnP-UGG_p4 GAAGAACGGAATCACGCTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 287-289 bp 1.0 83.6
trnW-CCA-trnP-UGG_p5 AGAAGAACGGAATCACGCTC ATTGAAGGGATGTAGCGCAG 50.0 50.0 58.0 58.0 328-330 bp 1.0 83.5