Hotspot

psbE-petL

LSC · 67152-68461 · 1310 bp

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Pi 0.0472
MarkerSeek score 77.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1310
variable_sites
60
pi
0.0472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AAGCCGCTAGTAGAAAACCA 50.0 45.0 57.8 56.9 1380-1451 bp 1.0 75.1
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AGCCGCTAGTAGAAAACCAA 50.0 45.0 57.8 56.9 1379-1450 bp 1.0 75.1
psbE-petL_p3 GTTCTCCTGTGCTTCCAGAC AAGCCGCTAGTAGAAAACCA 55.0 45.0 58.2 56.9 1375-1446 bp 1.0 74.8
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AGCCGCTAGTAGAAAACCAA 55.0 45.0 58.2 56.9 1374-1445 bp 1.0 74.8
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT AGCCGCTAGTAGAAAACCAA 50.0 45.0 58.4 56.9 1372-1443 bp 1.0 73.6