Hotspot

trnT-UGU-trnL-UAA

LSC · 48714-49413 · 700 bp

Back to Nasa

Pi 0.0773
MarkerSeek score 82.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
700
variable_sites
54
pi
0.0773
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 750-1015 bp 1.0 83.9
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC ACCAATTTCGCCATATCCCC 55.0 50.0 58.2 58.0 781-1046 bp 1.0 83.5
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 758-1023 bp 1.0 83.3
trnT-UGU-trnL-UAA_p4 GAGTCGAACCGATGACCATC TAGCGTCTACCAATTTCGCC 55.0 50.0 58.2 58.1 789-1054 bp 1.0 83.0
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 764-1029 bp 1.0 81.9