Hotspot

trnY-GUA-trnE-UUC

LSC · 32774-32832 · 59 bp

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Pi 0.0847
MarkerSeek score 78.20
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
5
pi
0.0847
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCAGCGTAGACATATTGCCA CATAGTATGATGGCGGTCGG 50.0 55.0 57.8 58.0 224-231 bp 1.0 79.8
trnY-GUA-trnE-UUC_p2 ACCAGCGTAGACATATTGCC CATAGTATGATGGCGGTCGG 50.0 55.0 57.8 58.0 225-232 bp 1.0 79.8
trnY-GUA-trnE-UUC_p3 TAATTATTGGGCCGAGCTGG CATAGTATGATGGCGGTCGG 50.0 55.0 57.7 58.0 251-258 bp 1.0 79.4
trnY-GUA-trnE-UUC_p4 CCAGCGTAGACATATTGCCA AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 202-209 bp 1.0 79.3
trnY-GUA-trnE-UUC_p5 ACCAGCGTAGACATATTGCC AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 203-210 bp 1.0 79.2