Hotspot

petN-psbM

LSC · 30123-31244 · 1122 bp

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Pi 0.049
MarkerSeek score 86.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1122
variable_sites
55
pi
0.049
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACATTTTCCCTTTCGCTCGT TGCTACTGCACTGTTCATTCT 45.0 42.9 58.1 57.6 1246-1263 bp 1.0 71.4
petN-psbM_p2 CCCTTTCGCTCGTAGTATGG TGCTACTGCACTGTTCATTCT 55.0 42.9 57.9 57.6 1239-1256 bp 1.0 71.2
petN-psbM_p3 CCTTTCGCTCGTAGTATGGG TGCTACTGCACTGTTCATTCT 55.0 42.9 57.9 57.6 1238-1255 bp 1.0 71.2
petN-psbM_p4 CTTTCGCTCGTAGTATGGGG TGCTACTGCACTGTTCATTCT 55.0 42.9 57.9 57.6 1237-1254 bp 1.0 71.2
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1284-1301 bp 1.0 70.9