Hotspot

ycf1

IRa · 128471-133819 · 5349 bp

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Pi 0.0224

MarkerSeek score 55.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 5349
variable_sites: 302
pi: 0.0224
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCGCCACTTTTTCGCTACT	TATTGCAATTCCCCGAGTGG	45.0	50.0	58.1	57.9	1239-1347 bp	0.857143	80.1
ycf1_p2	TTCGCCACTTTTTCGCTACT	GGAAGGGAAGCAGAACTAGC	45.0	55.0	58.1	58.0	985-994 bp	0.571429	65.9
ycf1_p3	AGTTGTGCATTGATCCTCGG	GGAAGGGAAGCAGAACTAGC	50.0	55.0	58.3	58.0	629-638 bp	0.571429	65.7
ycf1_p4	TTCGCCACTTTTTCGCTACT	GTCTGAAACCTTGGCGAAGA	45.0	50.0	58.1	58.1	1086-1095 bp	0.571429	64.8
ycf1_p5	TTCGCCACTTTTTCGCTACT	AACTAGCTTTTGGGTCTGCC	45.0	50.0	58.1	58.1	981 bp	0.142857	44.0