Hotspot

trnT-UGU-trnL-UAA

LSC · 48050-49050 · 1001 bp

Back to Narcissus

Pi 0.0357

MarkerSeek score 76.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 88
pi: 0.0357
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	TAAAACCTGGCCTGTTGACC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	1637-1704 bp	1.0	88.9
trnT-UGU-trnL-UAA_p2	CGAAGCCATACCCAATCGAA	ACCAATTTCGCCATATCCCC	50.0	50.0	58.1	58.0	1694-1761 bp	1.0	88.4
trnT-UGU-trnL-UAA_p3	TAAAACCTGGCCTGTTGACC	GATAGCCAGTTACCAACGCA	50.0	50.0	58.0	58.0	1865-1932 bp	1.0	88.1
trnT-UGU-trnL-UAA_p4	CGAAGCCATACCCAATCGAA	GATAGCCAGTTACCAACGCA	50.0	50.0	58.1	58.0	1922-1989 bp	1.0	87.6
trnT-UGU-trnL-UAA_p5	TAAAACCTGGCCTGTTGACC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	1645-1712 bp	0.857143	81.1