Hotspot

petN-psbM

LSC · 30021-30880 · 860 bp

Back to Narcissus

Pi 0.0217

MarkerSeek score 69.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 47
pi: 0.0217
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAAATGA	55.0	35.0	58.1	53.9	909-1019 bp	1.0	52.8
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAAATGA	55.0	35.0	58.1	53.9	908-1018 bp	1.0	52.8
petN-psbM_p3	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAAATGA	55.0	35.0	57.8	53.9	907-1017 bp	1.0	52.4
petN-psbM_p4	AGTATGGGGAAGGAGTGGAC	TGTAAAAACAGTCAGCCAAAATGA	55.0	33.3	58.1	57.8	913-1023 bp	1.0	52.0
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	TGTAAAAACAGTCAGCCAAAATGA	55.0	33.3	58.1	57.8	912-1022 bp	1.0	52.0