Hotspot

trnC-GCA-petN

LSC · 28870-29930 · 1061 bp

Back to Narcissus

Pi 0.0268

MarkerSeek score 71.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 69
pi: 0.0268
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	CAGCCCAAGCAAGACTTACT	50.0	50.0	57.5	57.8	1100-1146 bp	1.0	82.5
trnC-GCA-petN_p2	CCAGTTCAAATCCGGGTGTC	CAGCCCAAGCAAGACTTACT	55.0	50.0	58.8	57.8	1077-1123 bp	1.0	80.2
trnC-GCA-petN_p3	TATCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	57.0	57.8	1082-1128 bp	1.0	78.6
trnC-GCA-petN_p4	AATTTATTTGGCGGCATGGC	CAGCCCAAGCAAGACTTACT	45.0	50.0	58.0	57.8	1169 bp	0.142857	43.8
trnC-GCA-petN_p5	AATTTATTTGGCGGCATGGC	GCAGCCCAAGCAAGACTTA	45.0	52.6	58.0	58.1	1170 bp	0.142857	36.9