Hotspot

atpF-atpH

LSC · 14027-14522 · 496 bp

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Pi 0.0289

MarkerSeek score 67.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 496
variable_sites: 33
pi: 0.0289
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CATATTGTGCCCGGACTTCA	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.0	58.0	1041-1122 bp	0.857143	82.8
atpF-atpH_p2	CATATTGTGCCCGGACTTCA	AAGCCGTAGAAGGTATTGCG	50.0	50.0	58.0	58.1	1133-1214 bp	0.857143	82.2
atpF-atpH_p3	CATATTGTGCCCGGACTTCA	CAATATAAAATGCGCGCGGT	50.0	45.0	58.0	58.0	1458-1555 bp	0.857143	81.7
atpF-atpH_p4	CATATTGTGCCCGGACTTCA	CCAATTGAGGCAATGCATGG	50.0	50.0	58.0	58.1	1685-1800 bp	0.857143	81.6
atpF-atpH_p5	CATATTGTGCCCGGACTTCA	CCCAATTGAGGCAATGCATG	50.0	50.0	58.0	58.1	1686-1801 bp	0.857143	81.6