Hotspot

rps4

LSC · 1-633 · 633 bp

Back to Naravelia

Pi 0.00316

MarkerSeek score 46.00

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 633
variable_sites: 2
pi: 0.00316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AAAAATACGCCGTCTGGGT	AAAACCTGACCTGTTGACCC	47.4	50.0	57.4	57.9	246 bp	1.0	66.7
rps4_p2	AAAAATACGCCGTCTGGGT	TTGACCCTTTGGCTTTTCCA	47.4	45.0	57.4	57.8	233 bp	1.0	65.9
rps4_p3	AAAAATACGCCGTCTGGGT	CCTGACCTGTTGACCCTTTG	47.4	55.0	57.4	58.4	242 bp	1.0	64.1
rps4_p4	AAAAATACGCCGTCTGGGT	CTGACCTGTTGACCCTTTGG	47.4	55.0	57.4	58.4	241 bp	1.0	64.1
rps4_p5	AAAAATACGCCGTCTGGGT	GTTGACCCTTTGGCTTTTCC	47.4	50.0	57.4	57.5	234 bp	1.0	63.0