Hotspot

ccsA

SSC · 114860-115825 · 966 bp

Back to Naravelia

Pi 0.00207

MarkerSeek score 51.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 2
pi: 0.00207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCAGATCTCCATTCCATTCTTGG	TCAAAAACTCGTACAAACTTTCGT	43.5	33.3	58.0	57.8	1098-1100 bp	1.0	40.1
ccsA_p2	TCAGATCTCCATTCCATTCTTGG	CAAAAACTCGTACAAACTTTCGT	43.5	34.8	58.0	56.5	1097-1099 bp	1.0	40.1
ccsA_p3	TCAGATCTCCATTCCATTCTTGG	AAACTCGTACAAACTTTCGT	43.5	35.0	58.0	53.5	1094-1096 bp	1.0	40.1
ccsA_p4	TCAGATCTCCATTCCATTCTTGG	AACTCGTACAAACTTTCGTT	43.5	35.0	58.0	53.5	1093-1095 bp	1.0	40.1
ccsA_p5	CAGATCTCCATTCCATTCTTGG	TCAAAAACTCGTACAAACTTTCGT	45.5	33.3	56.6	57.8	1097-1099 bp	1.0	40.1