Hotspot

ndhE-ndhG

SSC · 118526-118813 · 288 bp

Back to Naravelia

Pi 0.0145

MarkerSeek score 65.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 288
variable_sites: 4
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AAGACACATAAGCGCTCGAA	TGCTATGGCTCGTCAGTACT	45.0	50.0	57.6	58.2	399-412 bp	1.0	78.6
ndhE-ndhG_p2	AGACACATAAGCGCTCGAAC	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.4	58.2	398-411 bp	1.0	78.5
ndhE-ndhG_p3	CAAGACACATAAGCGCTCGA	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.4	58.2	400-413 bp	1.0	78.5
ndhE-ndhG_p4	TCAAGACACATAAGCGCTCG	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.4	58.2	401-414 bp	1.0	78.5
ndhE-ndhG_p5	GCTCAAGACACATAAGCGCT	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.6	58.2	403-416 bp	1.0	76.3