Hotspot

ccsA-ndhD

SSC · 115826-116065 · 240 bp

Back to Naravelia

Pi 0.0292

MarkerSeek score 64.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 7
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGCTGCATAGTTATGGTTCA	TCCGGATTTCGTTCTCTCAC	47.6	50.0	58.1	57.4	352-354 bp	1.0	67.8
ccsA-ndhD_p2	GGGCTGCATAGTTATGGTTCA	CCGGATTTCGTTCTCTCACT	47.6	50.0	58.1	57.3	351-353 bp	1.0	67.8
ccsA-ndhD_p3	AGGGCTGCATAGTTATGGTTC	TCCGGATTTCGTTCTCTCAC	47.6	50.0	57.8	57.4	353-355 bp	1.0	66.7
ccsA-ndhD_p4	AGGGCTGCATAGTTATGGTTC	CCGGATTTCGTTCTCTCACT	47.6	50.0	57.8	57.3	352-354 bp	1.0	66.6
ccsA-ndhD_p5	AGGGCTGCATAGTTATGGTT	TCCGGATTTCGTTCTCTCAC	45.0	50.0	56.6	57.4	353-355 bp	1.0	65.0