Hotspot

accD-psaI

LSC · 58827-59373 · 547 bp

Back to Naravelia

Pi 0.00548

MarkerSeek score 60.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 3
pi: 0.00548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAGCTTCACGGTTTCTGTC	GCCATTGCAATTGCTGGAAA	50.0	45.0	58.1	58.2	655-659 bp	1.0	77.8
accD-psaI_p2	TTCAGCTTCACGGTTTCTGT	GCCATTGCAATTGCTGGAAA	45.0	45.0	57.7	58.2	656-660 bp	1.0	76.2
accD-psaI_p3	GCTTCACGGTTTCTGTCCTT	GCCATTGCAATTGCTGGAAA	50.0	45.0	58.4	58.2	652-656 bp	1.0	75.6
accD-psaI_p4	TCAGCTTCACGGTTTCTGTC	GAAGCCATTGCAATTGCTGG	50.0	50.0	58.1	58.6	658-662 bp	1.0	74.2
accD-psaI_p5	TCAGCTTCACGGTTTCTGTC	GGAAGCCATTGCAATTGCTG	50.0	50.0	58.1	58.6	659-663 bp	1.0	74.2