Hotspot

psaA-ycf3

LSC · 46473-47214 · 742 bp

Back to Naravelia

Pi 0.0027

MarkerSeek score 60.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 2
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGATGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	58.0	58.0	920 bp	1.0	79.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGATGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	58.0	57.9	935 bp	1.0	79.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGATGT	CGATCAAGCTGCCGAGTATT	50.0	50.0	58.0	58.1	922 bp	1.0	78.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGATGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.0	58.2	853 bp	1.0	78.3
psaA-ycf3_p5	GAGAAATGACCTGGTCTGGC	ATCAAGCTGCCGAGTATTGG	55.0	50.0	58.3	58.0	938 bp	1.0	77.8