Hotspot

atpH-atpI

LSC · 17369-18483 · 1115 bp

Back to Naravelia

Pi 0.0027

MarkerSeek score 60.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1115
variable_sites: 3
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGGTCCAATAGAAGCCA	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1215-1220 bp	1.0	79.7
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.1	57.9	1170-1175 bp	1.0	79.2
atpH-atpI_p3	GATACCTTCTACGGCTTGGC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.1	57.9	1257-1262 bp	1.0	78.9
atpH-atpI_p4	CGATACCTTCTACGGCTTGG	ATAGGGGAATCCATGGAGGG	55.0	55.0	57.9	57.9	1258-1263 bp	1.0	78.9
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	1188-1193 bp	1.0	78.8