Hotspot

ndhJ-ndhK

LSC · 11129-11263 · 135 bp

Back to Naravelia

Pi 0.0148

MarkerSeek score 61.00

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 2
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GCCAATCCTCGGGCTTTATT	CGCCATCTACTTCAGAGATCC	50.0	52.4	58.3	57.7	324 bp	1.0	67.7
ndhJ-ndhK_p2	CAAGCAGACAAACGACCTTG	CGCCATCTACTTCAGAGATCC	50.0	52.4	57.6	57.7	238 bp	1.0	67.2
ndhJ-ndhK_p3	CCAAGCAGACAAACGACCTT	CGCCATCTACTTCAGAGATCC	50.0	52.4	58.7	57.7	239 bp	1.0	64.8
ndhJ-ndhK_p4	GCCAATCCTCGGGCTTTATT	GCCATCTACTTCAGAGATCCC	50.0	52.4	58.3	57.3	323 bp	1.0	64.6
ndhJ-ndhK_p5	CAAGCAGACAAACGACCTTG	GCCATCTACTTCAGAGATCCC	50.0	52.4	57.6	57.3	237 bp	1.0	64.1