Hotspot

ycf1

SSC · 116533-121965 · 5433 bp

Back to Nanocnide

Pi 0.0302

MarkerSeek score 55.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5433
variable_sites: 163
pi: 0.0302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTTCTTAGGGTTCAATTCGC	TCCTGAATGGTCCGAGGATT	47.6	50.0	57.2	58.1	1226-1244 bp	1.0	69.7
ycf1_p2	GGTTCTTAGGGTTCAATTCGC	TTCCTGAATGGTCCGAGGAT	47.6	50.0	57.2	58.1	1227-1245 bp	1.0	69.7
ycf1_p3	GGTTCTTAGGGTTCAATTCGC	CCGAGGATTTAACGGAGTGG	47.6	55.0	57.2	58.1	1233 bp	0.5	45.4
ycf1_p4	ACAAAGTGACTCTGCGATAGT	CCGAGGATTTAACGGAGTGG	42.9	55.0	57.1	58.1	1007 bp	0.5	43.9
ycf1_p5	ACAAAGTGACTCTGCGATAGT	TCCTGAATGGTCCGAGGATT	42.9	50.0	57.1	58.1	1018 bp	0.5	43.5