Hotspot

rpl16

LSC · 75471-76828 · 1358 bp

Back to Nanocnide

Pi 0.0359

MarkerSeek score 71.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1358
variable_sites: 46
pi: 0.0359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AACGTCACCAACTTGAGCAT	AGGGAAGGTAAAGTGCCTCT	45.0	50.0	58.0	58.0	1803-1874 bp	1.0	85.8
rpl16_p2	TTCAGGCGCTAATGAAACGA	AGGGAAGGTAAAGTGCCTCT	45.0	50.0	57.9	58.0	2049-2120 bp	1.0	85.3
rpl16_p3	AACGTCACCAACTTGAGCAT	TGCACGTGTTGAATGGATCA	45.0	45.0	58.0	58.1	1822-1893 bp	1.0	85.3
rpl16_p4	AACGTCACCAACTTGAGCAT	CAGGGAAGGTAAAGTGCCTC	45.0	55.0	58.0	57.9	1804-1875 bp	1.0	85.3
rpl16_p5	AACGTCACCAACTTGAGCAT	ACGTGTTGAATGGATCAGGG	45.0	50.0	58.0	57.9	1819-1890 bp	1.0	85.2