Hotspot

ndhD

SSC · 108345-109874 · 1530 bp

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Pi 0.0131

MarkerSeek score 51.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 20
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ATGCAGCTCTTTTATGCGGA	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	2481-2485 bp	1.0	81.5
ndhD_p2	TGCAGCTCTTTTATGCGGAT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	2480-2484 bp	1.0	81.5
ndhD_p3	ATGCAGCTCTTTTATGCGGA	GGGACGGGTGTAAAGCTAAG	45.0	55.0	58.0	58.0	2510-2514 bp	1.0	81.5
ndhD_p4	TGCAGCTCTTTTATGCGGAT	GGGACGGGTGTAAAGCTAAG	45.0	55.0	58.0	58.0	2509-2513 bp	1.0	81.5
ndhD_p5	GAGCAGTATGGGCCAATGAA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	2193-2197 bp	1.0	81.4