Hotspot

ccsA-ndhD

SSC · 108153-108344 · 192 bp

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Pi 0.115

MarkerSeek score 75.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 192
variable_sites: 22
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGGTTACATAGTTATGGTTCA	AGTTGACAAAGTCGAAGCTCT	36.4	42.9	54.2	57.5	275-278 bp	1.0	46.4
ccsA-ndhD_p2	AGGGTTACATAGTTATGGTTCA	TCAGTTGACAAAGTCGAAGCT	36.4	42.9	54.2	57.8	277-280 bp	1.0	46.3
ccsA-ndhD_p3	AGGGTTACATAGTTATGGTTCA	CAGTTGACAAAGTCGAAGCTC	36.4	47.6	54.2	57.5	276-279 bp	1.0	46.3
ccsA-ndhD_p4	AGGGTTACATAGTTATGGTTCAT	TCAGTTGACAAAGTCGAAGCT	34.8	42.9	54.8	57.8	277-280 bp	1.0	46.3
ccsA-ndhD_p5	AGGGTTACATAGTTATGGTTCA	TCAGTTGACAAAGTCGAAGC	36.4	45.0	54.2	56.3	277-280 bp	1.0	46.3