Hotspot

rpl33-rps18

LSC · 62410-62648 · 239 bp

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Pi 0.1

MarkerSeek score 77.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 22
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACGCCTAGTCGATTGGAATT	AGCCGCTTGGATTTAACCAT	45.0	45.0	57.0	57.9	337 bp	1.0	80.2
rpl33-rps18_p2	ACGCCTAGTCGATTGGAATT	AGAGCCGCTTGGATTTAACC	45.0	50.0	57.0	58.2	339 bp	1.0	79.3
rpl33-rps18_p3	CGCCTAGTCGATTGGAATTGA	AGCCGCTTGGATTTAACCAT	47.6	45.0	58.2	57.9	336 bp	1.0	78.7
rpl33-rps18_p4	CGCCTAGTCGATTGGAATTGA	AGAGCCGCTTGGATTTAACC	47.6	50.0	58.2	58.2	338 bp	1.0	77.8
rpl33-rps18_p5	CGCCTAGTCGATTGGAATTG	AGCCGCTTGGATTTAACCAT	50.0	45.0	56.7	57.9	336 bp	1.0	77.6