Hotspot

psaJ-rpl33

LSC · 61785-62208 · 424 bp

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Pi 0.0334

MarkerSeek score 61.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 14
pi: 0.0334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGTACCCAAAACAAACGCG	TAAGGCGGTTTCGAACACAA	45.0	45.0	57.8	58.0	1092-1097 bp	1.0	80.9
psaJ-rpl33_p2	TTGTACCCAAAACAAACGCG	TTAAGGCGGTTTCGAACACA	45.0	45.0	57.8	58.0	1093-1098 bp	1.0	80.9
psaJ-rpl33_p3	TGGCACCCGTAGTAAGTACT	TAAGGCGGTTTCGAACACAA	50.0	45.0	57.8	58.0	601-610 bp	1.0	80.7
psaJ-rpl33_p4	TGGCACCCGTAGTAAGTACT	TTAAGGCGGTTTCGAACACA	50.0	45.0	57.8	58.0	602-611 bp	1.0	80.7
psaJ-rpl33_p5	TGGCATTGTAGAGACTCCCT	TAAGGCGGTTTCGAACACAA	50.0	45.0	57.7	58.0	1033-1038 bp	1.0	80.4