Hotspot

trnW-CCA-trnP-UGG

LSC · 61034-61155 · 122 bp

Back to Nanocnide

Pi 0.0796

MarkerSeek score 77.00

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 9
pi: 0.0796
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	58.0	57.8	214-215 bp	1.0	84.9
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	TTGAAAGGGATGTAGCGCAG	50.0	50.0	58.0	58.3	242-243 bp	1.0	83.5
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	TAGCGCGTTTGTTTTGGGTA	50.0	45.0	58.0	58.4	217-218 bp	1.0	83.1
trnW-CCA-trnP-UGG_p4	ACCGAACTGAACTAAGAGCG	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.7	57.8	194-195 bp	1.0	83.1
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	ATTGAAAGGGATGTAGCGCA	50.0	45.0	58.0	57.6	243-244 bp	1.0	82.3