Hotspot

petA-psbJ

LSC · 57889-58705 · 817 bp

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Pi 0.0465

MarkerSeek score 79.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 37
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAAATCTTTTTGGTTCT	ATTCTGGATTAGGGTCCTCC	36.4	50.0	57.2	55.7	897-914 bp	1.0	45.1
petA-psbJ_p2	TGGCACAAATCTTTTTGGTTCT	ACTGGTATTCTTGTGATCGGT	36.4	42.9	57.2	56.7	946-963 bp	1.0	44.9
petA-psbJ_p3	TGGCACAAATCTTTTTGGTTCT	TCTTGTGATCGGTTTAATCGGA	36.4	40.9	57.2	57.5	938-955 bp	1.0	44.9
petA-psbJ_p4	TGGCACAAATCTTTTTGGTTCT	AACTGGTATTCTTGTGATCGGT	36.4	40.9	57.2	57.4	947-964 bp	1.0	44.9
petA-psbJ_p5	TGGCACAAATCTTTTTGGTTCT	ACTGGTATTCTTGTGATCGGTT	36.4	40.9	57.2	57.4	946-963 bp	1.0	44.9